Isolation and identification of a pepsitensin.
نویسندگان
چکیده
1. From ox plasma incubated with pepsin a highly purified pepsitensin has been isolated by fractional precipitation, solvent extraction, column chromatography, countercurrent distribution and paper chromatography. 2. Comparison of the properties of this substance with those of synthetic valyl-5 angiotensin I showed: (a) the same specific pressor activity; (b) the same amino acid composition; (c) identical paper-electrophoretic mobilities at various pH values. 3. N- and C-Terminal studies carried out on the intact polypeptide and on the products of chymotrypsin digestion established the following sequence for the amino acids present in the molecule: Asp-Arg-Val-Tyr-Val-His-Pro-Phe-His-Leu. This structure is identical with that of valyl-5 angiotensin I.
منابع مشابه
A pressor material produced by the action of pepsin on casein.
Hypertensin is a pressor polypeptide which may be important in renal hypertension (1). It is formed by the action of renin on a plasma globulin (2). Attempts to obtain a related substance by using systems better adapted to preparative work were initially unpromising (2), but Croxatto et al. (3,4) later isolated “pepsitensin,” a pressor material, after incubation of pepsin with several proteins....
متن کاملIsolation and identification of Aeromonas hydrophila from an outbreak of haemorrhagic septicemia in snakes
متن کامل
Isolation, identification and antimicrobial susceptibility of pathogenic Aeromonas media isolated from diseased Koi carp (Cyprinus carpio koi)
Aeromonas infections are the most common bacterial disease of cultured fish in China. In this study, a gram-negative bacillus was isolated from the liver of diseased koi carp (Cyprinus carpio koi), and named strain KC-2. The results of morphological and biochemical tests, as well as phylogenetic analysis derived from 16S rRNA and gyrB sequences indicated that the isolated strain KC-2 was highly...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- The Biochemical journal
دوره 97 2 شماره
صفحات -
تاریخ انتشار 1965